Microcosm study: Laboratory measurements of biodegradation

In this project, Macondo surrogate and heavy crude oil (bitumen) underwent biodegradation in a microcosm for 0-15 days. Both fractionated to yield structurally defined fractions: saturates, 1-ring, 2-ring, 3-ring, 4-ring, and 5+ring aromatics. The fractions with acceptable yields underwent biodegradation as well, to determine each structural class's contribution to the transformation products. Products were analyzed for DOC (dissolved organic carbon), and by Fourier-transform ion cyclotron resonance mass spectrometer (FT-ICR MS) for molecular-level changes. This dataset supports the publication: Niles, S. F., Chacón-Patiño, M. L., Chen, H., McKenna, A. M., Blakney, G. T., Rodgers, R. P., & Marshall, A. G. (2019). Molecular-Level Characterization of Oil-Soluble Ketone/Aldehyde Photo-Oxidation Products by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Reveals Similarity Between Microcosm and Field Samples. Environmental Science & Technology, 53(12), 6887–6894. doi:10.1021/acs.est.9b00908

本研究中，将马孔多替代油（Macondo surrogate）与重质原油（bitumen，沥青）置于微生物微宇宙体系中，开展为期0至15天的生物降解实验。随后将两种样品分别进行分馏分离，得到结构明确的组分：饱和烃、单环芳烃、双环芳烃、三环芳烃、四环芳烃以及五环及以上芳烃。将收率合格的各组分再次开展生物降解实验，以明确每一类结构组分对降解转化产物的贡献。对所得降解产物分别检测溶解有机碳（dissolved organic carbon, DOC）含量，并通过傅里叶变换离子回旋共振质谱（Fourier-transform ion cyclotron resonance mass spectrometer, FT-ICR MS）表征其分子层面的组成变化。本数据集支撑以下已发表学术论文：Niles, S. F., Chacón-Patiño, M. L., Chen, H., McKenna, A. M., Blakney, G. T., Rodgers, R. P., & Marshall, A. G. (2019). 基于傅里叶变换离子回旋共振质谱的油溶性酮/醛光氧化产物分子级表征揭示微宇宙与野外样品的相似性. 环境科学与技术（Environmental Science & Technology）, 53(12), 6887–6894. doi:10.1021/acs.est.9b00908