Expression data from E12 mouse lumbar motor neuron subsets

Sonic hedgehog (Shh) signals via Gli transcription factors to direct digit number and identity in the vertebrate limb. We have characterized the Gli-dependent cis-regulatory network through a combination of whole genome ChIP-on-chip and transcriptional profiling of the developing mouse limb. In this dataset, we include the expression data obtained from dissected mouse forelimbs using a variety of gain- and loss-of-function hedgehog pathway mutants, as well as limbs dissected into responsive (posterior 2/3ds) and non-responsive (anterior 1/3d) Hh tissues. These data are used to obtain 753 genes that are differentially expressed in response to Shh signaling. 6 Total samples were analyzed. We generated the following pairwise comparisons using PowerExpress: medial MN vs lateral MN FDR <= x % and a fold-change >= 1.5 were selected.音猬因子（Sonic hedgehog, Shh）通过Gli转录因子（Gli transcription factors）介导信号传导，以调控脊椎动物肢体的指骨数量与身份特化。本研究结合全基因组染色质免疫沉淀-芯片（whole genome ChIP-on-chip）与发育中小鼠肢体的转录组分析，解析了Gli依赖的顺式调控网络。本数据集包含来自解剖获取的小鼠前肢的表达数据：实验样本涵盖多种功能获得（gain-of-function）与功能缺失（loss-of-function）型刺猬信号通路突变体，同时将解剖后的肢体划分为响应性（后2/3区域）与非响应性（前1/3区域）Hh组织。通过上述数据，我们筛选出753个响应Shh信号通路的差异表达基因。本次分析共纳入6个样本，使用PowerExpress软件构建了如下成对比较：筛选错误发现率（False Discovery Rate, FDR）≤x%且折叠变化（fold-change）≥1.5的内侧MN与外侧MN样本。