Evaluation of liver regeneration diet supplemented with omega-3 fatty acids: experimental study in rats

Objective: to evaluate liver regeneration in rats after partial hepatectomy of 60% with and without action diet supplemented with fatty acids through the study of the regenerated liver weight, laboratory parameters of liver function and histological study. Methods: thirty-six Wistar rats, males, adults were used, weighing between 195 and 330 g assigned to control and groups. The supplementation group received the diet by gavage and were killed after 24h, 72h and seven days. Evaluation of regeneration occurred through analysis of weight gain liver, serum aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidase, and mitosis of the liver stained with H&E. Results: the diet supplemented group showed no statistical difference (p>0.05) on the evolution of weights. Administration of fatty acids post-hepatectomy had significant reduction in gamma glutamyltransferase levels and may reflect liver regeneration. Referring to mitotic index, it did not differ between period of times among the groups. Conclusion: supplementation with fatty acids in rats undergoing 60% hepatic resection showed no significant interference related to liver regeneration.

研究目的：本研究通过检测再生肝重量、肝功能实验室指标及组织学分析，评估补充脂肪酸膳食与普通膳食对大鼠60%部分肝切除术后肝再生的影响。研究方法：选用36只成年雄性Wistar大鼠，体重介于195~330g，随机分为对照组与脂肪酸补充膳食组。脂肪酸补充膳食组经灌胃给予添加脂肪酸的膳食，两组大鼠分别于术后24h、72h及7d处死。通过肝增重分析、血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶、γ-谷氨酰转移酶水平测定，以及苏木精-伊红（H&E）染色后肝脏有丝分裂观察，对肝再生情况进行评估。研究结果：与对照组相比，脂肪酸补充膳食组的肝重量变化无统计学差异（p>0.05）。肝切除术后给予脂肪酸膳食可显著降低血清γ-谷氨酰转移酶水平，该变化或可反映肝再生进程。而就有丝分裂指数而言，两组间各时间点的检测结果均无显著差异。研究结论：对实施60%肝切除术的大鼠补充脂肪酸膳食，未对其肝再生产生显著干扰作用。