Effect of ROQUIN2(Y691F) expression on mRNA levels upon BCR stimulation

Roquin2, and its paralog Roquin1, are key regulator of mRNA decay. Roquin proteins recognize mRNAs containing a 3'-UTR stem-loop motif, called constitutive decay element (CDE), through the ROQ domain. Upon target engagement, Roquin proteins recruit the CCR4-CAF1-NOT complex, mediating mRNA deadenylation and destabilization. To study the effect of Roquin2 stabilization, we sought out to identify the relevant Roquin2 mRNA targets in human DLBCL. To this purpose, we measured differential gene expression via RNA-seq analysis in U-2932 cells expressing Roquin2 or non-degradable Roquin2 upon BCR crosslinking. Overall design: U-2932 stably expressin Roquin2(WT) or Roquin2(Y691F) were treated with F(ab')2-IgM at 10 µg/ml for 12 hours. RNA was collected from three independent biological replicates per condition.