We used the CRISPR Off-Target Tool (http://www.mhi-humangenetics.org/en/resources/) to score candidate sgRNAs.

sgRNA group: Reason of sgRNA inclusion. SNP/Gene: Targeted locus. sgRNA_ID: The sgRNA ID. sgRNA_Seq: The sgRNA sequence. Targeting_guide_score: This score assumes that the sgRNA should have one genomic match that is the intended target. If there is no perfect match, this score will be equal to the non-targeting score. Non_targeting_score: This score assumes that the sgRNA should NOT match any sequence of the genome. best_match_score: Score of best match. best_match_position: Position of best match. best_match_sequence: Sequence of best match. best_match_CFD: CFD (cutting frequency determination) score of best match. mean_CFD: Mean CFD score. median_CFD: Median CFD score. max_CFD: Maximum CFD score. min_CFD: Minimum CFD score. perc_CFD: 10, 25, 75 and 90th percentile of CFD score. Total_matches: Total number of matches. N_X: These columns contain the number of matches with X mismatches. There will be X+1 columns ranging from N_0 (perfect match) to N_X, where X is the maximum number of allowed mismatches. Genomic positions are on build hg19.
(XLSX)