High spatial resolution multi-omics atlas sequencing of mouse embryos

Microfluidic deterministic barcoding of mRNAs and proteins in tissue slides followed by high throughput sequencing enables the construction of high-spatial-resolution multi-omics atlas at the genome scale. Applying it to mouse embryo tissues revealed major tissue (sub)types in early-stage organogenesis, brain micro-vasculatures, and the fine structure of an optical vesicle at the single-cell-layer resolution. Applying new approach--microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing to mouse embryo tissues in different resolution (10um, 25um, 50um).对组织切片中的mRNA和蛋白质开展微流控确定性条形码标记（microfluidic deterministic barcoding），结合后续高通量测序，可构建基因组尺度的高空间分辨率多组学图谱。将该技术应用于小鼠胚胎组织后，在单细胞层分辨率下揭示了早期器官发生过程中的主要组织（亚）类型、脑部微血管系统以及光学囊泡的精细结构。本研究将面向空间组学测序的组织内微流控确定性条形码标记新技术（microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing）应用于不同分辨率（10μm、25μm、50μm）的小鼠胚胎组织。