Expression profiles in multiple tissues of WT and Nrf2-knockout mouse under space flight

To elucidate roles that Nrf2 plays in regulating adaptive responses to the space stress, one month-long space travel and RNA-seq analysis of six Nrf2-KO mice and six wild-type mice were conducted . After 31-days-stay in the ISS, all the flight mice returned safely to Earth. We found that Nrf2 was indeed activated by the space stress in various tissues. The space stress and Nrf2-deficiency brought about changes in gene expression. mRNA profiling using multiple tissues including temporal bone, mandibular bone, spleen, liver, white and brown adipose tissues, kidney, thymus, and brain cerebellum of wild type (WT) and Nrf2-knockout (Nrf2KO) mice为阐明核因子红细胞2相关因子2（Nrf2）在调控机体针对太空应激的适应性应答中所扮演的角色，本研究开展了为期1个月的太空飞行实验，并对6只Nrf2敲除（Nrf2-KO）小鼠与6只野生型小鼠进行RNA测序（RNA-seq）分析。所有实验小鼠在国际空间站（ISS）驻留31天后，均安全返回地球。研究结果显示，太空应激确实可在多种组织中激活Nrf2；太空应激与Nrf2缺失均可引发基因表达改变。本研究针对野生型（WT）与Nrf2敲除（Nrf2KO）小鼠的多种组织开展mRNA表达谱分析，所涉组织包括颞骨、下颌骨、脾脏、肝脏、白色脂肪组织与棕色脂肪组织、肾脏、胸腺以及大脑小脑。