Human Knee Cartilage Histopathology Assessment

90 patients with varus knees scheduled for total knee arthroplasty were recruited after getting their informed consent. Inclusion criteria required patients with a diagnosis of idiopathic OA (primarily medial compartment and/or patellofemoral disease) exhibiting a relatively preserved lateral compartment (JSW: 2–10 mm, median: 6 mm in the lateral compartment) based on preoperative weight-bearing anterior-posterior (AP) radio- graphs taken in full extension and 30° of flexion. During TKA, the Lateral Femoral Condyle (LFC) was collected and the AP orientation was noted. All included LFC specimens presented with Grade 0 , I , or II macroscopic Outerbridge classifications. Two osteochondral specimens (4 × 4 × 8 mm) were obtained from each LFC by placing the condyle in an in-house fabricated miter box in AP orientation and cartilage arches were cut using a razor blade from the weight-bearing center portion of the LFC; 1 was located medial and 1 lateral to the LFC midline. The centers of these two samples were separated by 10 mm.

A total of 180 osteochondral specimens were processed from 90 patients. Immediately after surgical retrieval, specimens were fixed for 48 h at 4 °C. 5µ thick paraffin sections were cut and stained with freshly prepared HE or SafraninO and fast green (SafO). Two adjacent sections per stain were digitally imaged under brightfield at 5x and used for scoring using HHGS and advanced OARSI systems. The HHGS and OARSI scores were provided by 3 reviewers, 3 times (separated by at least 3 months). Unstained sections were digitally imaged under polarized light microscopy (PLM) at 1.25X and scored using PLM scoring system.

In addition to the digital high quality tiff images of SafO, HE and PLM sections of cartilage and the respective HHGS, OARSI and PLM scores, metadata would also include patient's age, gender, surgery-side.

本研究共纳入90名拟行全膝关节置换术（Total Knee Arthroplasty, TKA）的膝内翻患者，所有受试者均签署知情同意书。纳入标准为：经术前完全伸直位及屈曲30°位负重前后位（Anterior-Posterior, AP）X线片证实为特发性骨关节炎（Osteoarthritis, OA），且外侧间室关节间隙宽度（Joint Space Width, JSW）相对保留（外侧间室JSW范围2~10 mm，中位数为6 mm），病变主要累及内侧间室和/或髌股关节。术中采集股骨外侧髁（Lateral Femoral Condyle, LFC）标本并记录其前后位取向，所有纳入的LFC标本宏观Outerbridge分级均为0、I或II级。

针对每个LFC，将其按前后位取向放置于自制斜切夹具中，从其负重中心区域取材制备2块骨软骨标本（尺寸为4×4×8 mm）：使用剃须刀切割软骨瓣，两块标本分别位于LFC中线的内侧及外侧，两样本中心间距为10 mm。本研究共从90例患者中获取180份骨软骨标本。

标本于术中获取后立即置于4℃环境下固定48小时。制作厚度为5μm的石蜡切片，采用新鲜配制的苏木精-伊红（Hematoxylin-Eosin, HE）染色液或番红O-固绿（SafraninO and fast green, SafO）染色液进行染色。每种染色剂取两张相邻切片，在明场显微镜下以5倍放大倍率进行数字化成像，采用HHGS评分系统及改良OARSI评分系统（Osteoarthritis Research Society International, OARSI）进行评分。由3名评审员分别进行3次独立评分（每次评分间隔至少3个月），最终获得HHGS与OARSI评分结果。未染色切片在偏振光显微镜（Polarized Light Microscopy, PLM）下以1.25倍放大倍率进行数字化成像，采用偏振光评分系统进行评分。

本数据集除包含番红O染色、HE染色及偏振光显微镜成像的软骨高清数字化TIFF图像，以及对应的HHGS、OARSI与偏振光评分外，元数据还将涵盖患者年龄、性别及手术侧别。