Arid1a promotes tumor initiation but restrains progression and metastasis in liver cancer [RNA-seq]. Mus musculus

ARID1A is hypothesized to be one of the most common tumor suppressors in human cancer. However, the functional consequences of ARID1A gain and loss are not clear. We report that mice with complete liver-specific Arid1a deficiency were resistant to MYC induced liver carcinogenesis, indicating a requirement during tumor initiation. Mechanistically, Arid1a loss constrained tumor formation by reducing Cytochrome P450 expression, which in turn mitigated oxidative stress. We also examined the heterozygous state and found it potentiated metastasis formation and heterozygous has similar gene expression pattern with homozygous compared to WT. Overall design: Liver tumor tissues were collected from LAP-MYC; Arid1a WT,Arid1a Het and Arid1a Homo knockout mice, then transcriptional profiling were generated by RNA-seq analysis.Liver tissues were collected from Rag1-/-; LAP-MYC; Arid1a WT with ad-cre or ad-ARID1A overexpression mice, then transcriptional profiling were generated by RNA-seq analysis.