GLs .xlsx

Glucosinolate content was analyzed using 4 weeks old plants. Five plants of each line were used as biological replicates. Plants were cut from top of the soil, put in liquid nitrogen to stop chemical reactions and transferred to freeze-drier for 3 days. Freeze-dried samples were finely ground using micro-dismemberator U (B. Braun Biotech International). Finely grounded material was sent to NIOO for dedicated glucosinolate analysis. Briefly, samples were boiled in 70% MeOH followed by washing two times in 1 ml of 70% MeOH and two times with 1 mL MQ water and one times in 2mL of NaOAC 16 hr ahead of treatment with 20 μl arylsulfatase. Following arylsulfatase treatment freeze dried elute was redissolved in 1mL MQ water and filtered over a 20 μm filter. Separation and detection of glucosinolates were performed using reversed phase C-18 column and photodiode array detector respectively. Glucosinolates measurement and concentration calculation was done following the procedure of Kabouw et al., (2010). A list of measured glucosinolates and their abbreviated names are given in