Expression data from uterine tissue of Egr1 knockout mice.

Microarrays have been widely used to study estrogen responses, as estrogen-induced transcriptome changes significantly impact the uterus. This study investigates the gene expression profiling of Egr1 knockout (KO) mice treated with estrogen. Our goal is to identify transcriptomic features that differ between estrogen-treated Egr1 knockout uteri and wild-type (WT) uteri. We examined the gene expression patterns of 17 samples, consisting of Egr1 KO and WT uteri, at different time points: before estrogen treatment (0 h) and after treatment at 3 h, 6 h, and 24 h, using the MouseWG-6 V2 platform. Our findings indicate that Egr1 KO uteri exhibit dysregulation in reproductive and immune systems compared to WT uteri, and we observed altered insulin growth factor and PARylation activity related to uterine proliferation. Microarrays were initially performed by using total uterine RNA. Uterine tissues were collected at 0, 3, 6, and 24 h after E2 treatment. The Illumina Mouse WG-6 V2 (48 K) array (Illumina, San Diego, CA, USA) was hybridized with appropriate cRNA probes at ISTECH (Goyang, South Korea). Expression values and detection calls were computed from the raw data. <<< Submitter states that raw (non-normalized) data is unavailable for this submission due to circumstances beyond their control >>>