DAXX interacts with histone acylation in gene regulation

Using proteome arrays and mass spectrometry, we find that the histone chaperone DAXX interacts with crotonylated histones. We show that histone crotonylation and gene expression changes promoted by the short-chain fatty acid crotonate depend on DAXX. RNA-Seq and pan-lysine crotonylation (Kcr, PTM-501) ChIP-Seq experiments from the colon epithelium-derived HCT116 cells, where DAXX or non-targeting control small interfering RNA were used to knock down DAXX or be used as control. Then cells were either treated or not with 10 mM crotonate for 18 h. All RNA-seq experiments were performed in quadruplicate.