Histone H4 lysine 20 mono-methylation directly facilitates chromatin 'openness' and promotes transcription of housekeeping genes (ATAC-seq_DRB)

To study the role of Histone H4 lysine 20 mono- and tri-methylation (H4K20me1, H4K20me3) in relationship to chromatin accessibility and gene expression, we report the application of high-throughput profiling of these marks in synchronized and asynchronous Human Osteosarcoma U2OS and asynchronous Mouse Fibroblast (MEF) cells with Set8 knockdown or control siRNA treatment. We find that H4K20me1 contributes to the open chromatin structure at active genes. Overall design: siRNA knockdown of the H4K20 methyltransferase Set8 in humans cells followed by ATAC-seq of DRB-treated cells.