Microsatellites markers used in this study.

Characteristics of the microsatellite markers developed for Avicennia germinans previously developed [22; 23; 24] and for A. schaueriana, described herein. The expected size based on the clone fragment, GenBank accession number, repeat motif, optimal PCR amplification conditions (AC) are shown for each marker. TD65-55 indicates touchdown PCR with temperatures ranging from 65 to 55°C. P indicates polymorphic and M denotes monomorphic marker for A. germinans (Ag) and A. schaueriana (As). * indicates evidence of null alleles for three or more samples. Microsatellites markers used in this study.