Evaluation of therapeutic efficacy of Sodium thiosulfate in combating I/R injury in rat brain: Exploring its efficiency further in vascular calcified brain slice model

Cerebral ischemia reperfusion injury (CIR) is one of the clinical manifestations encountered during the management of stroke. High prevalence of intracranial arterial calcification is reported in stroke patients. However, the impact of vascular calcification (VC) in the outcome of CIR and therapeutic interventions remains unclear. Two different experimental models namely carotid artery occlusion and brain slice models were used to evaluate the efficacy of STS in male Wistar rats. IR was inflicted in rat by occluding carotid artery for 30 minutes followed by 24 hr reperfusion after STS (100 mg/kg) administration. Brain slice model was used to reconfirm the results to account blood brain barrier permeability. Further, brain slice tissue was utilised to evaluate the efficacy of STS in VC rat brain by measuring the histological alterations and biochemical parameters.