Microarray analysis of Wild-type vs. Brpf1-deficient E8.75 mouse embryo

RNA was isolated from three pairs of wild-type and Brpf1-deficient E8.75 mouse embryos for microarray analysis. The cDNA samples were then labeled and mixed for hybridization with one array. The data from three arrays were obtained and used for bioinformatic analysis.为开展微阵列分析（microarray analysis），我们从3对野生型与Brpf1基因缺陷型E8.75期小鼠胚胎中分离提取总RNA。随后将所得互补DNA（complementary DNA, cDNA）样本进行标记并混合，与单张微阵列芯片完成杂交反应。最终获得3张微阵列芯片的检测数据，并将其应用于生物信息学分析。