MMGC: custom Kraken2/Bracken database for analysing the mouse gut microbiome

Custom Kraken2/Bracken database built using the representative genomes for 1,021 microbial species from the mouse gut microbiota. Genomes include isolates and MAGs, but all are near-complete (>90% completeness; <5% contamination; maximum genome size ≤ 8 Mb; maximum contig count ≤ 500; N50 ≥ 10 kb; mean contig length ≥ 5 kb). This database achieved a mean read classification rate of 87.7% when benchmarked on 1,785 independent (i.e. non-contributory) mouse gut shotgun metagenome samples. An equivalent human database (UHGG) only attained classification rates of 36.6%. This database is a publicly available resource to facilitate more efficient/deeper analyses of mouse gut shotgun metagenomes. Find out more about the Mouse Microbial Genome Collection at our GitHub repository.