Multi-dimensional transcriptome analysis of an intact hematopoietic organ for HSPC expansion [RNA-seq]

To decode the complexed mechanism controlling HSC expansion, from the viewpoint of systems biology, we performed spatial transcriptome analysis by dissecting the whole hematopoietic organ, CHT. All the reagents and instruments used in this experiment are RNase-free. The fresh 52 hpf zebrafish tail was embedded in OCT with vertical position and rapid frozen in liquid nitrogen. The cryosection was stained with cresyl violet before laser microdissection. Each sample of sections was acquired using specific LCM platforms. The tail region of 52 hpf zebrafish embryo was cut for cryo-section and six sets of samples (including neuro (N), left muscle (L), right muscle (R), caudal artery (CA), caudal vein (CV) and caudal vein plexus (CVP)) were harvested from each section by Laser Capture Microdissection (LCM).