scRNA-seq of in vitro differentiated D6 mPGCLCs

Germ cell specification is an elusive biological event, as it occurs in a small group of cells in the very early embryo (E6.5). In vitro differentiation of naive mouse Embryonic Stem Cells (mESCs) to mouse Primordial Germ Cell like cells (mPGCLCs) allows the mechanistic investigation of germ cell specification. The goal of this study is to profile of gene expression via single cell RNA sequencing throughout the differentiation protocol. In particular, this project aims at examining the transcriptional activation of the molecular networks that direct the epigenetic reprogramming of mPGCLCs. Single cell RNAseq profiling (10x Genomics) of mESc during in vitro differentiation to mPGCLCs. One replicate for the bulk cell cultures (mESCs and mEPILCs), three biologial replicates for the Embryoid bodies at day 6 (EBd6) each derived from one individual dissociated EB