Leptospira rLRR20 stimulates gene expressions in HK2 cell

Leptospires are highly motile bacteria that migrate from the breached skin to blood circulation of in human, allowing for their rapid dissemination and subsequent colonization of the liver, lungs, and kidneys. Pathogenic Leptospira contained numerous leucine-rich repeat (LRR) genes compared to non-pathogenic species that acted as virulence factors. The functions of the LRR proteins are still unknown and the relative responses of the host cell provided clear evidence that the regulation of host cell during Leptospirosis. We used microarrays to observation the global gene expression of human kidney epithelial cell (HK2 cell) under the treatment of rLRR20 protein. HK2 cell were cultured to 70% confluence and serum-starved overnight before stimulating with 10 μM purified rLRR20 for 4 h. The control groups were treated with PBS buffer. The total RNA were extracted and hybridization on Affymetrix microarrays.