Effects of increased intracellular calcium and overnutrition on pancreatic beta cell transcriptome

To determine the individual and combined effects of excitotoxicity and overnutrition on beta-cell gene expression, we analyzed the impact of a high fat diet (HFD) on wild type C57Bl/6J and Abcc8 knockout mice of different sexes. We collected 31 bulk RNA-Seq datasets that originated from FACS-purified pancreatic beta-cells that differed by genotype, diet, and sex.为明确兴奋性毒性（excitotoxicity）与营养过剩各自及联合效应对胰腺β细胞基因表达的影响，本研究分析了高脂饮食（HFD）对不同性别野生型C57Bl/6J小鼠与Abcc8基因敲除小鼠的影响。我们共收集了31份批量RNA测序（bulk RNA-Seq）数据集，这些数据源自经荧光激活细胞分选（FACS）纯化的、基因型、饮食条件及性别均存在差异的胰腺β细胞。