Randomized PAM depletion of phylogenetically-diverse Cas12a nucleases
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE130377
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资源简介：
We report the PAMs of phylogenetically-diverse Cas12a nucleases using a cell-free TXTL-based PAM screen. By adding a 5N randomized PAM library and Cas12a-gRNA in vitro, recognized PAM sequences were cleaved, while non-recognized PAMs remained. By amplifying the non-cleaved DNA, we used next-generation sequencing to analyze the depletion of functional PAMs of these Cas12a nucleases. Uncleaved DNA from the randomized PAM library was amplified using Illumina Nextera Indices
创建时间：
2020-06-08
