RNA-seq of TGF-beta1-induced NRK-52E cells after treatment with 2D- and 3D-EVs

Renal tubular epithelial cells are the main cells affected in the process of renal fibrosis, and it has been shown that mesenchymal stem cell-derived exosomes can treat renal fibrosis, and then the traditional 2D-EVs production method is inefficient. We efficiently extracted 3D-EVs by coaxial bioprinting, but the mechanism of 3D-EVs for treating renal fibrosis is not yet known. Therefore, we used TGF-beta1 to induce a renal fibrosis phenotype in NRK-52E cells and took 2D- and 3D-EVs for treatment in order to explore the possible differential molecular mechanisms of 2D- and 3D-EVs for renal fibrosis. This approach facilitates the discovery of new mechanisms for exosomes to treat renal fibrosis. To compare the difference in efficacy between 2D-EVs of conventional methods and efficiently produced 3D-EVs in the treatment of renal fibrosis. We induced a renal fibrosis phenotype in rat renal tubular epithelial cells NRK-52E by TGF-beta1, followed by treatment with 2D- and 3D-EVs.