Single-cell sequencing reveals homogeneity and heterogeneity in the molecular cytopathological mechanisms of AKI caused by different etiologies

We constructed single-cell atlases of AKI induced by different etiologies, revealed potential homogeneity of injury patterns and heterogeneity between models in AKI. Extensive cell death and cytoskeletal remodeling events in proximal tubular cells (PTCs) as well as aberrant proliferation and inflammation of endothelial cells (ECs) in AKI are common injury patterns across different AKI models. However, there are differences in injury outcomes in PTCs with different etiologies, and ECs also have distinct transcriptional profiles. Single-cell sequenceing of AKI induced by different etiologies: cisplatin (CP), folic acid (FA), sodium oxalate (SO), ischemia reperfusion injury (IRI), and unilateral ureteral obstruction (UUO).