Cytokine secretion in wild type and LRRK2 KO BMDM’s following LPS stimulation.

Bone marrow derived macrophages from LRRK2 knockout mice and wild type littermate controls were plated at a density of 1×106 in 2 ml of tissue culture media. Cells were treated with 100 ng/ml LPS 24 h after plating and media collected at the indicated time points. Cytokines were measured with an antibody based multiplex assay and concentrations determined by standard curve. Data is presented as mean concentration in pg/ml ± S.E.M. Experiments were performed with n = 4 in duplicate.