Comparison of RNA modification profiles between Primary Human T-Cells and Immortalized Jurkat Cells

We used Nanopore direct RNA sequencing to identify and compare m6A, inosine, and pseudouridine modifications in poly(A) RNA derived from primary human T-Cells and immortalized Jurkat cells. We analyzed these modifications across the whole genome and at a site-specific level. We documented that the modification landscapes were conserved between these two cell types, with Pseudouridine having the largest variation. We also compared differential RNA expression profiles of modification machinery associated genes using RNA-seq data from the Human Protein Atlas. We documented that RNA modification machinery genes were mostly stable in expression levels across paired immortalized cell lines and conjugate cell types. Our differential expression analysis showed a stable RNA modification machinery associated gene expression pattern when compared to randomly sampled genes.