Investigation of the effect of silencing PARP2 by shRNA in human keratinocytes

PARP2 was knocked-down in HPV-Ker human keratinocytes by the application of a PARP2-specific short hairpin shRNA-containing lentiviral vector that resulted in approximately 80 % decrement in PARP2 protein expression in shP2 HPV-Ker cells compared to the sc cells transduced with a negative control sequence-containing lentivirus. To characterize the role of PARP2 in keratinocyte inflammatory processes, we stimulated sc and shP2 HPV-Kers with a psoriasis-mimicking cytokine cocktail, the combination of IL17A and TNFa, and performed RNA-sequencing from vehicle and cytokine -treated sc and shP2 cells, respectively.