Additional file 14 of Using RNA-Seq for gene identification, polymorphism detection and transcript profiling in two alfalfa genotypes with divergent cell wall composition in stems

Additional file 14:300 housekeeping genes selected. A table listing 300 housekeeping genes (HKGs) with relatively high levels of expression. To identify these HKGs, we first selected genes with an average RPKM-normalized transcript count greater than 10. Next, we selected the top 300 genes from the list with the lowest coefficient of variation (CV = standard deviation/mean). The RPKM-normalized expression counts, MapMan functional class and description for each HKG selected are also presented in the table. (TXT 76 KB)

附加文件14：筛选得到300个持家基因。该表格列出了300个表达水平相对较高的持家基因（housekeeping genes, HKGs）。为筛选得到这些HKGs，本研究首先选取了平均经RPKM（Reads Per Kilobase per Million mapped reads）标准化后的转录计数大于10的基因；随后从该列表中挑选出变异系数（coefficient of variation, CV=标准差/均值）最低的前300个基因。表格中同时呈现了每个所选HKGs的RPKM标准化表达计数、MapMan功能分类及对应描述。（TXT格式，76 KB）