Purification of β-lactamase-SBP using various streptavidin matrices.
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*Theoretical binding capacity is expressed in terms of the amounts (µg) of β-lactamase-SBP that can bind to the matrix. This value is estimated with the assumption that one SBP tag binds to two subunits in a tetrameric streptavidin. 1 µg of streptavidin dimer (Mr = 33,038) will bind 1.06 µg of β-lactamase-SBP (Mr = 35,030.6). New matrix was used in each binding study.**Amount recovered is based on the total amount of pure proteins recovered in selected wash fractions and the elution fractions.
创建时间：
2015-12-02
