The complete mitochondrial genome of Engyodontium album and comparative analyses with Ascomycota mitogenomes

Abstract Engyodontium album is a widespread pathogen that causes different kinds of dermatoses and respiratory tract diseases in humans and animals. In spite of its perniciousness, the basic genetic and molecular background of this species remains poorly understood. In this study, the mitochondrial genome sequence of E. album was determined using a high-throughput sequencing platform. The circular mitogenome was found to be 28,081 nucleotides in length and comprised of 17 protein-coding genes, 24 tRNA genes, and 2 rRNA genes. The nucleotide composition of the genome was A+T-biased (74.13%). Group-II introns were found in the nad1, nad5, and cob genes. The most frequently used codon of protein-coding genes was UAU. Isoleucine was identified as the most common amino acid, while proline was the least common amino acid in protein-coding genes. The gene-arrangement order is nearly the same when compared with other Ascomycota mitogenomes. Phylogenetic relationships based on the shared protein-coding genes revealed that E. album is closely related to the Cordycipitaceae family, with a high-confidence support value (100%). The availability of the mitogenome of E. album will shed light on the molecular systematic and genetic differentiation of this species.

摘要 白被毛孢（Engyodontium album）是一种分布广泛的病原菌，可引发人类与动物的多种皮肤病及呼吸道疾病。尽管该菌具有致病性，但目前学界对其基础遗传与分子背景的认知仍较为匮乏。本研究借助高通量测序平台，完成了白被毛孢线粒体基因组序列的测定。该环状线粒体基因组全长28081个核苷酸，包含17个蛋白质编码基因、24个转运RNA（tRNA）基因以及2个核糖体RNA（rRNA）基因。该基因组的核苷酸组成具有A+T偏好性（74.13%）。在nad1、nad5及cob基因中发现了II类内含子（Group-II introns）。蛋白质编码基因最常用的密码子为UAU。在蛋白质编码基因中，异亮氨酸为最常见的氨基酸，而脯氨酸为最不常见的氨基酸。与其他子囊菌门（Ascomycota）线粒体基因组相比，其基因排列顺序几乎一致。基于共有蛋白质编码基因构建的系统发育关系显示，白被毛孢与虫草菌科（Cordycipitaceae）亲缘关系密切，且具有100%的高置信支持值。白被毛孢线粒体基因组的公开，将为该物种的分子系统学与遗传分化研究提供新的见解。