Concatenation of alignments from the 50 single-gene datasets with highest relative tree certainty scores recovers Rhodelphis as sister to red algae.

A maximum likelihood phylogenetic tree was generated with IQ-TREE based on a concatenated alignment of the 50 single gene trees with the highest relative tree certainty (151 taxa, 21,886 sites). The sister relationship of Rhodelphis and red algae still receives full statistical support with a highly reduced, phylogenetically well-supported dataset. This dataset represents Extended Data Figure 6.

基于相对树确定性最高的50棵单基因树的串联比对序列，使用IQ-TREE构建了最大似然系统发育树（包含151个分类单元，共计21886个比对位点）。借助该精简且在系统发育层面得到充分支持的数据集，Rhodelphis与红藻的姐妹群关系仍获得了完全的统计支持。该数据集对应扩展数据图6。