Effects of 9,10-phenanthrenequione on antioxidant indices and metabolite profiles in Takifugu obscurus plasma

Derived from polycyclic aromatic hydrocarbons (PAHs), oxygenated-PAHs (oxy-PAHs) may pose hazards to aquatic organisms, which remains largely unknown. Takifugu obscurus is an important anadromous fish species of high economic and ecological values. In the present study, T. obscurus was acutely exposed to 44.29 μg/L 9,10-phenanthrenequione (9,10-PQ) for 96 hours. Changes of antioxidant indices and metabolite profiles in plasma were compared between 9,10-PQ treatment and the control. The results showed that 9,10-PQ treatment significantly increased MDA content during 6 hours to 96 hours, increased SOD and CAT activities at 6 hours, but decreased them at 96 hours. These results indicated that 9,10-PQ induced oxidative stress to fish. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) analysis revealed that four metabolic pathways were influenced in response to treatment with 9,10-PQ, including glycerophospholipid metabolism, phenylalanine, tyrosine and tryptophan biosynthe...

氧代多环芳烃（oxygenated-PAHs, oxy-PAHs）源自多环芳烃（polycyclic aromatic hydrocarbons, PAHs），其可能对水生生物造成危害，但目前相关认知仍较为匮乏。暗纹东方鲀（Takifugu obscurus）是兼具高经济与生态价值的重要溯河洄游鱼类。本研究将暗纹东方鲀以44.29 μg/L的9,10-菲醌（9,10-phenanthrenequione, 9,10-PQ）进行96小时急性暴露，对比了9,10-PQ处理组与对照组血浆中的抗氧化指标及代谢物谱变化。结果显示，9,10-PQ处理可在6小时至96小时期间显著提升血浆丙二醛（malondialdehyde, MDA）含量；在暴露6小时时，超氧化物歧化酶（superoxide dismutase, SOD）与过氧化氢酶（catalase, CAT）活性上升，但在96小时时二者活性出现下降。上述结果表明，9,10-PQ可诱导鱼类产生氧化应激。超高效液相色谱-质谱联用（ultra-performance liquid chromatography-mass spectrometry, UPLC-MS）分析显示，9,10-PQ处理共影响了4条代谢通路，包括甘油磷脂代谢、苯丙氨酸、酪氨酸与色氨酸生物合成……