Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

研究目的 本研究评估了四种pH调节方式处理椰子水（coconut water，CW）后，对人成纤维细胞（human fibroblasts，HFF）存活率的影响。
材料与方法 本研究使用氢氧化钠（Sodium Hydroxide，NaOH）、碳酸氢钠（Sodium bicarbonate，NaHCO₃）、三乙醇胺（Triethanolamine，C₆H₁₅NO₃）、2-氨基-2-甲基-1-丙醇（2-Amino-2-Methyl-1-Propanol，C₄H₁₁NO）四种调节剂，将天然及工业化椰子水的pH值调节至7.0。将成纤维细胞以2×10⁴个/孔的密度接种于96孔板中，于上述椰子水溶液中分别孵育2小时及4小时。阳性对照为使用DMEM培养基培养的HFF，阴性对照为使用自来水培养的HFF。采用MTT甲瓒分析法进行细胞存活率检测。数据采用三因素方差分析（3-way ANOVA），随后辅以Tukey检验与Dunnet检验进行多重比较。
结果 无论椰子水类型、孵育时长，或是椰子水类型与孵育时长的交互作用、椰子水类型与pH调节方式的交互作用、pH调节方式与孵育时长的交互作用，对细胞存活率均无显著影响（p>0.05）。
结论 用于椰子水pH调节的试剂并未对HFF的存活率产生影响，但天然椰子水存在表现更优的趋势。