Effect of salinity acclimation on mitochondrial respiration of soft shell clam Mya arenaria

Salinity change in coastal zone is a common phenomena which can put several coastal soft bottom organisms under stressed condition. These stress could be mediated through their mitochondrial functions. In this data set, we present the effect of acclimation to three different salinity regime (normal: 15 psu, low: 5 psu and fluctuating: daily salinity cycle between 5 and 15 psu) on the mitochondrial performance (MO2) of soft shell clam, Mya arenaria. Clams were acclimated to normal, low and fluctuating salinity; the mitochondrial respiration of these clams was then measured under two different osmolarity (450 and 150 mOsm, representing 15 and 5 psu, respectively). MO2 was measured sequentially, starting with the addition of pyruvate and malate, succinate, ADP, oligomycin, CCCP, rotenon, antimycin A, TMPD and ascorbate, and finally KCN.

滨海区域盐度变化是一种常见现象，可使多种滨海软底生物陷入胁迫状态。此类胁迫可通过生物体的线粒体功能实现介导。本数据集呈现了三种不同盐度驯化条件[正常盐度：15 psu，低盐度：5 psu，波动盐度：每日盐度在5至15 psu间循环]对软壳蛤（Mya arenaria）线粒体性能（MO2）的影响。实验将软壳蛤分别驯化于正常、低盐及波动盐度环境，随后在两种渗透压条件（450和150 mOsm，分别对应15和5 psu）下测定其线粒体呼吸速率。MO2的测定按序进行，依次添加丙酮酸盐（pyruvate）与苹果酸盐（malate）、琥珀酸盐（succinate）、腺苷二磷酸（ADP）、寡霉素（oligomycin）、羰基氰化物间氯苯腙（CCCP）、鱼藤酮（rotenon）、抗霉素A（antimycin A）、N,N,N',N'-四甲基对苯二胺（TMPD）与抗坏血酸盐（ascorbate），最终添加氰化钾（KCN）。