Gene expression profiling of AGS Gastric Cancer cells upon YY1 knock down

Yin Yang 1 (YY1) is a ubiquitous transcription factor belonging to the GLI-Kruppel class of zinc finger proteins regulating diverse biological processes including cell proliferation, development, DNA damage responses and also involved in carcinogenesis. The protein is involved in repressing and activating a diverse number of promoters.In order to study the role of the transcription factor YY1 in gastric cancer, transcriptome wide gene expression analysis was performed upon silencing the YY1 gene in gastric cancer cell line, AGS using Affymetrix Human Transcriptome 2.0 array. Transcriptome Analysis Console (TAC) Software was used for the analysis of differentially expressed genes between AGS_YY1_siRNA transfected and AGS_NTC_siRNA transfected cells. Total RNA was extracted from AGS gastric cancer cells transfected with YY1siRNA and NTC siRNA and profiled using GeneChip® Human Transcriptome Array 2.0 (HTA-2_0).