Quality control in the different stages of producing red blood cell concentrate from dogs

ABSTRACT The objective of this study was to perform a quality control assessment of red blood cells after standardization of the blood production stages. For this purpose, separation of the blood components to obtain red blood cells, the storage of the blood packets and an evaluation of blood quality were performed. The mean (± SD) volume, globular volume, hemoglobin and hemolysis percentage of the red blood cell concentrate were 299.77±30.08mL, 60.87±2.60%, 20.57±0.93g/DL and 0.09±0.07%, respectively. The means (± SD) of the volume, globular volume, total hemoglobin percentage of hemolysis and hemoglobin per unit of packed red blood cells after the storage period (8.83±6.73 days) were 57.55±3.01%, 20.30±0.89 0, 20±0.12%, and 60.90±7.65. The red blood cell packets were within the parameters of quality control established by Health Ministry legislation in humans and allow us to conclude that the standardization of blood production stages involves the selection of donors until the end of storage and is necessary to produce quality red blood cells. Quality control aims to find possible flaws in the procedures to be repaired, increasing transfusion safety.

摘要
本研究旨在对血液生产流程标准化后的红细胞开展质量控制评估。为此，本研究完成了血液成分分离以获取红细胞、血液袋储存以及血液质量评估相关工作。浓缩红细胞（red blood cell concentrate）的平均值（±标准差）体积、红细胞比容、血红蛋白含量及溶血率分别为299.77±30.08mL、60.87±2.60%、20.57±0.93g/dL及0.09±0.07%。储存期（8.83±6.73天）后，每单位压积红细胞（packed red blood cells）的体积、红细胞比容、总血红蛋白、溶血率及血红蛋白含量分别为57.55±3.01%、20.30±0.89、0、20±0.12%及60.90±7.65。本研究涉及的红细胞储存袋符合人类卫生部制定的人类用血质量控制参数标准，由此可得出结论：血液生产流程标准化涵盖从献血者筛选直至储存结束的全环节，且对制备合格红细胞而言是必要的。质量控制旨在识别流程中可被修正的潜在缺陷，以此提升输血安全性。