Supplementary Data for "Long-term pollution does not significantly inhibit denitrification and DNRA by adapted benthic microbial communities"

<strong>Title:</strong> Long-term pollution does not significantly inhibit denitrification and DNRA by adapted benthic microbial communities <br> <strong>Supplementary Information</strong> <em>Captions to supplementary data files</em> <br> Data S1 The data file shows sequence facility ids, sample names, number of read-pairs yielded after sequencing, quality trimming and merging details, number of 16S rRNA gene sequences classified against SILVA, 18S rRNA gene sequences against NCBI NT, and meagenome sequences classified with DIAMOND against the MEGAN supplied KEGG database. <br> Data S2 The data file shows the denitrification and DNRA results from the IPT experiment. <br> Data S3 The data file shows: sheet 1) KEGG classifications in the nitrogen metabolism pathway, and sheet 2) all the KEGG classifications for all pathways detected in th samples. The table shows normalized reads counts derived from the MEGAN software normalized read counts function (sub-sample to lowest sample size). <br> Data S4 The data file shows MEGAN taxonomy linked to KEGG classifications (metal related genes, Dioxin pathway, and Nitrogen metabolism pathways). The results are based on linking taxonomy and KEGG classifications having the same read name. The table shows normalized counts as counts per million (CPM) values. <br> Data S5 The data file shows the edgeR results (only results with FDR &lt; 0.05 are reported). The table shows log fold-change values based on pairwise comparisons between stations. <br> Data S6 The data file shows 16S rRNA gene sequences (sheet 1) extracted from the metagenome (using SortMeRNA) and classified against the SILVA database. The table shows raw read counts.

<strong>标题：</strong>长期污染不会显著抑制适应性底栖微生物群落的反硝化作用（denitrification）与异化硝酸盐还原为铵（DNRA，Dissimilatory Nitrate Reduction to Ammonium） <br> <strong>补充信息</strong> <em>补充数据文件说明</em> <br> 数据S1 本数据文件包含测序机构编号、样本名称、测序后经质量修剪与合并的读对（read-pairs）数量详情、针对SILVA数据库分类的16S核糖体RNA（16S rRNA）基因序列数、针对NCBI NT数据库分类的18S核糖体RNA（18S rRNA）基因序列数，以及通过DIAMOND比对MEGAN提供的KEGG数据库进行分类的宏基因组序列数。 <br> 数据S2 本数据文件包含IPT实验中的反硝化作用与DNRA结果。 <br> 数据S3 本数据文件包含：工作表1）氮代谢通路中的KEGG分类信息，以及工作表2）样本中检测到的所有通路的全部KEGG分类信息。该表格展示了通过MEGAN软件的标准化读计数功能（将样本抽样至最小样本量）得到的标准化读计数。 <br> 数据S4 本数据文件包含与KEGG分类信息关联的MEGAN分类学注释信息（涉及金属相关基因、二噁英通路及氮代谢通路）。该结果基于具有相同读名称的分类学与KEGG分类信息的关联得到。表格中的标准化计数以每百万读长计数（CPM，Counts Per Million）形式展示。 <br> 数据S5 本数据文件包含edgeR分析结果（仅报告错误发现率（FDR，False Discovery Rate）<0.05的结果）。表格展示了基于站点间两两比较得到的对数倍变化值。 <br> 数据S6 本数据文件包含从宏基因组中（通过SortMeRNA工具）提取并针对SILVA数据库分类的16S核糖体RNA（16S rRNA）基因序列（工作表1）。表格展示了原始读长计数。