CD81 marks immature and dedifferentiated pancreatic β-cells

We performed 3 single-cell RNAseq experiments to dissect the postnatal islet heterogeneity. In order to reach our goal, we used the Fltp lineage tracing (FltpiCre mTmG) mouse line. Thus we used mice at the age of 16 days, isolated islets of Langerhans from them and sorted via flow cytometry the three Fltp subpopulations. 3 single-cell RNA-seq experiments were performed using 10X Genomics technology to generate single cell transcriptomic profiles of pancreatic islets of Langherans. Cells were sorted to according to the fluorescence of our lineage tracing mouse model and processed separately.