Italian CryoEM Bag (ICB)

This BAG application builds on the development of single particle cryoEM in the Italian Structural Biology community, as in Italy there is no top-end instrument is open to rhe structural biology community.
The groups in this application have acquired know-how in cryoEM through international collaborations or by acquiring medium-level in-house equipment. Access to
the CM01 beamline at the ESRF since August 2020 has allowed the development of projects from groups that do not have in-house equipment that resulted in paper in publication on Nucleic Acid Research NAR.
We now have four screening microscopes in Italy and three more are being funded, making the access to CM01 more demanded and relevant for high-resolution data and, consequently high profile publications.

本BAG申请依托意大利结构生物学领域的单粒子冷冻电子显微镜（cryoEM）技术发展——鉴于意大利暂无面向结构生物学共同体开放的顶级冷冻电镜设备。
本次申请涉及的科研团队，已通过国际合作或购置中端自有设备的方式，掌握了冷冻电镜相关技术诀窍。自2020年8月起，借助欧洲同步辐射光源（ESRF）的CM01光束线开展实验，无自有冷冻电镜设备的科研团队得以推进相关研究项目，并在《核酸研究》（Nucleic Acid Research, NAR）发表了学术成果。
目前意大利境内已配备4台筛选级冷冻电镜，另有3台正处于获批资助阶段，这使得CM01光束线的使用需求愈发旺盛，其在获取高分辨率实验数据、进而发表高水平学术论文方面的价值也愈发凸显。