Slf1p and Sro9p affinity isolations

To identify RNAs specifically associated with Slf1p and Sro9p, cells expressing TAP-tagged Slf1, Sro9 or Fpr1 (negative control) were grown to mid-log phase in synthetic complete medium and harvested by centrifugation为鉴定与Slf1p和Sro9p特异性结合的RNA，将表达TAP标签（Tandem Affinity Purification）融合Slf1、Sro9或Fpr1（阴性对照）的细胞在合成完全培养基中培养至对数中期，随后通过离心收集细胞。