Mechanism of Yiqi Huoxue Granule in Promoting Angiogenesis by Increasing CSF2 to Reduce Mesenchymal Stem Cell Apoptosis

Objective: To explore the effect and mechanism of the Yiqi Huoxue Granule in improving the survival of mesenchymal stem cells (MSCs) induced by hypoxia and promoting angiogenesis in damaged tissues.Methods: Cell experiments: A hypoxic MSC model was constructed. The effect of the Yiqi Huoxue Granule on the viability of hypoxic MSCs was detected by CCK8. Flow cytometry was used to observe the apoptosis rate of MSCs. The effect of Yiqi Huoxue Granule on tube formation of MSCs was observed by tube formation assay. Differentially expressed genes were analyzed using transcriptomics and verified through RT-PCR. Key factors were analyzed by PPI.