Confetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics

Data from ProteomeXchange, PXD ID: PXD000900. Data file: HeLa_ArgC-Elastase_CID_2.mgf. Published as part of Mol Cell Proteomics. 2014 Apr 2 . From the Abstract: {{i}} ... In an attempt to improve protein sequence coverage and to target regions of proteins that do not generate useful tryptic peptides, we deployed a multi-protease strategy on the HeLa proteome. First, we used seven commercially available enzymes in single, double and triple enzyme combinations. A total of 48 digests were performed. 5223 proteins were detected by analyzing the unfractionated cell lysate digest directly, with 42% mean sequence coverage ... {{/i}}