MOESM1 of Asymmetric DNA methylation of CpG dyads is a feature of secondary DMRs associated with the Dlk1/Gtl2 imprinting cluster in mouse

Additional file 1. Data used for statistical analyses of DNA methylation levels at the Gtl2- and IG-DMRs. The numerical data used to perform Mann-Whitney U tests and the resulting P values are contained in this file. Data from the Gtl2-DMR 5’ region, Gtl2-DMR 3’ region and the IG-DMR are presented in separate sheets. Within each sheet, data from each of the developmental stages are presented in chronological order, as they are in the Results, Figures, and Tables. Each sheet presents the information for a specific locus, tissue, cross (maternal allele x paternal allele), and parental allele analyzed, as indicated in columns A-D. % methylation (column F) was calculated by dividing the number of methylated CpG sites observed in a given subclone (column E) by the total number of CpG sites analyzed within the subclone; the raw data used to make these calculations are found in Figs. 2, 3 and 4. For the Gtl2-DMR 3’ region and the IG-DMR, P values were calculated independently for BxC samples vs. CxB samples. In addition, P values were calculated for the combined BxC + CxB samples, as some of the BxC and CxB sample sizes were too small to accurately perform Mann-Whitney U tests. Data presented in sheets labelled “grouped” combine subclones derived from the same PCR with identical DNA methylation patterns and identical sequences as a single sample, while every subclone analyzed is treated as an independent sample in sheets labelled “ungrouped”.

附加文件1：用于Gtl2差异甲基化区域（Gtl2-DMR）与IG差异甲基化区域（IG-DMR）DNA甲基化水平统计分析的数据。本文件包含用于执行曼-惠特尼U检验（Mann-Whitney U test）的数值数据及所得P值。Gtl2-DMR 5'端区域、Gtl2-DMR 3'端区域及IG-DMR的数据分别置于不同工作表中。每个工作表内，各发育阶段的数据均按时间顺序排列，与正文结果、图表及表格中的呈现方式一致。每个工作表均包含特定分析的位点、组织、杂交组合（母本等位基因×父本等位基因）及亲本等位基因的相关信息，对应列A至列D。甲基化百分比（列F）通过将单个亚克隆（列E）中观测到的甲基化CpG位点数量除以该亚克隆内分析的总CpG位点数量计算得出；用于该计算的原始数据见于图2、图3及图4。针对Gtl2-DMR 3'端区域与IG-DMR，分别对BxC样本与CxB样本计算了P值。此外，由于部分BxC与CxB样本量过小无法准确执行曼-惠特尼U检验，还对合并后的BxC+CxB样本计算了P值。标记为"grouped"的工作表中，将源自同一PCR、具有相同DNA甲基化模式及相同序列的亚克隆合并为单个样本；而标记为"ungrouped"的工作表中，每个被分析的亚克隆均被视为独立样本。