Intravital microscopy datasets examining key nephron segments of transplanted decellularized kidneys

This study contains intravital microscopy (IVM) data examining the microarchitecture of acellular kidney scaffolds. Acellular scaffolds are cell-free collagen-based matrices derived from native organs that can be used as templates for regenerative medicine applications. This data set contains <em>in vivo</em> assays that evaluate the effectiveness of decellularization and how these acellular nephron compartments perform in the post-transplantation environment. Qualitative and quantitative assessments of scaffold DNA concentrations, tissue fluorescence signals, and structural and functional integrities of decellularized tubular and peritubular capillary segments were acquired and compared to the native (non-transplanted) organ. Cohorts of 2-3-month-old male Sprague Dawley rats were used: non-transplanted (n = 4), transplanted day 0 (n = 4), transplanted day 1 (n = 4), transplanted day 2 (n = 4), and transplanted day 7 (n = 4). Micrographs and supporting measurements are provided to illustrate IVM processes used to perform this study and are publicly available in a data repository to assist scientific reproducibility and extend the use of this powerful imaging application to analyze other scaffold systems.

本研究包含活体显微镜（intravital microscopy, IVM）数据，用于探究无细胞肾脏支架的微观结构。无细胞支架是源自天然器官的无细胞胶原基基质，可作为再生医学应用的模板。本数据集包含体内（in vivo）实验，用于评估脱细胞处理的效果，以及此类无细胞肾单位结构在移植后环境中的功能表现。研究对支架的DNA浓度、组织荧光信号，以及脱细胞后的肾小管和管周毛细血管段的结构与功能完整性开展了定性与定量评估，并将结果与天然（未移植）器官进行对比。实验采用2~3月龄的雄性斯普拉格·道利（Sprague Dawley）大鼠，分为以下组别：未移植组（n=4）、移植后0天组（n=4）、移植后1天组（n=4）、移植后2天组（n=4）及移植后7天组（n=4）。本研究提供了用于展示IVM实验流程的显微图像及配套测量数据，已公开至数据仓库，以助力科研结果可重复，并推广该先进成像技术在其他支架系统分析中的应用。