Eukaryotic microbial community at the LTER site Helgoland Roads from March 2016 to March 2019

Water samples were collected from the long-term ecological research (LTER) site at Helgoland and the eukaryotic microbial community was assessed. 18S V4 region was amplified using the primer set 528iF /964iR and amplicon sequencing was performed on an Illumina MiSeq™ sequencer in a 2 × 300 bp paired-end run. Sequence data have been deposited in the European Nucleotide Archive (ENA) at the European Bioinformatics Institute (EMBL-EBI) under accession number PRJEB37135 using the data brokerage service of the German Federation for Biological Data (GFBio). 21 million sequences remained after bioinformatic processing and were clustered into Operational Taxonomic Units (OTUs). The Protist Ribosomal Reference database (PR2), version 4.11.1 was used as reference database.

从赫尔戈兰岛的长期生态研究（Long-term Ecological Research, LTER）站点采集水样，对其中的真核微生物群落进行了表征分析。使用引物组合528iF/964iR扩增18S rRNA基因的V4区域，并在Illumina MiSeq™测序平台上以2×300 bp双端测序模式完成扩增子测序。通过德国生物数据联盟（German Federation for Biological Data, GFBio）的数据经纪服务，将测序数据提交至欧洲生物信息学研究所（EMBL-EBI）旗下的欧洲核苷酸档案库（European Nucleotide Archive, ENA），登录号为PRJEB37135。经生物信息学流程处理后保留了2100万条序列，并将其聚类为操作分类单元（Operational Taxonomic Units, OTUs）。本研究采用原生生物核糖体参考数据库（Protist Ribosomal Reference database, PR2）4.11.1版作为分类注释的参考数据库。