CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [ChIP-seq_set4]

In this experiment, we sought to identify how the distribution of H3K27me3 and H3K4me3 change in WT, MAZ HoxA(delta5|6), MAZ HoxD(delta4|8), and CTCF (delta5|6:6|7) cells during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs) Examination of H3K27me3 and H3K4me3 binding in embryonic stem cells and cervical motor neurons using ChIP-seq, by status of motif deletion in the cells: WT, MAZ HoxA(delta5|6), MAZ HoxD(delta4|8), and CTCF (delta5|6:6|7)