Mass spectrometry identification of peptides containing the sites of frameshifting.

The charge state (Charge), measured m/z value (m/z) and error of measurement in parts per million (Error; ppm) are given for the strongest detected peptide signal for each of the predicted frameshifting events. The scores for peptide identifications are given for both the SequestHT and Mascot search engines. The Mascot spectrum to peptide sequence assignments marked by an asterisk were not considered high confidence identifications, but also did not match any other sequence in the SwissProt sequence database (546,057 sequence entries) and therefore represent the most likely spectrum to sequence match. The peptide intensity is given as the area under the curve (peak area) for the extracted ion chromatogram of the strongest peptide signal of each frameshifting event and can be used as a rough estimation of peptide abundance. No CID: this low intensity signal was not selected for collision induced dissociation tandem mass spectrometry. The peptide detection is therefore based on high mass accuracy precursor mass measurement with an error of <1 ppm (+/−0.0016 Da).
Mass spectrometry identification of peptides containing the sites of frameshifting.