Additional file 2 of Dimethyl fumarate reduces TNF and Plasmodium falciparum induced brain endothelium activation in vitro

Additional file 2. Differentially expressed pathways in TNF treated HBMVEC compared to media treatment. HBMVEC incubated with TNF (10 ng/ml) or media alone for 6 h. Total RNA was extracted, reverse-transcribed, amplified and hybridized to a microarray. Gene expression profiles were generated using a robust multi-array average algorithm followed by quantile normalization and batch correction. Data represents three independent experiments, each done in triplicate. 41 Ingenuity Canonical Pathways were significantly differentially expressed with p value calculated by a right tailed Fisher’s exact test. (p

附加文件2：与培养基对照组相比，肿瘤坏死因子（Tumor Necrosis Factor，TNF）处理的人脑微血管内皮细胞（Human Brain Microvascular Endothelial Cells，HBMVEC）中差异表达的通路。将HBMVEC分别以10 ng/ml的TNF或单纯培养基孵育6小时。提取总RNA，经逆转录、扩增后与微阵列芯片进行杂交。采用稳健多阵列平均（robust multi-array average）算法生成基因表达谱，随后进行分位数归一化与批次校正。本数据源自3次独立实验，每次实验均设置3次重复。共有41条Ingenuity经典通路（Ingenuity Canonical Pathways）呈现显著差异表达，其P值通过右侧费舍尔精确检验计算得到。(p