Exon Array analysis of WT1(+KTS) mutant mice

This exon array analysis was performed on mice carrying splice specific mutations at the end of exon9 of the Wilms tumor supressor gene that interfere with the production of WT1(+KTS) isoforms (Hammes et al., 2001; Cell 106:309) 4 wildtype and 4 wt1(+KTS) mutant E18.5 mouse embryos were transcardially perfused with magnetic beads, glomeruli isolated and RNA extracted and analyzed for differential expression on Affymetrix Exon Arrays本研究针对携带威尔姆斯肿瘤抑制基因（Wilms Tumor Suppressor Gene, WT1）外显子9末端剪接特异性突变的小鼠开展外显子芯片（exon array）分析，该突变会干扰WT1(+KTS)亚型的产生（Hammes等人，2001；《Cell》106卷：309页）。我们对4只野生型小鼠和4只携带wt1(+KTS)突变的E18.5小鼠胚胎以磁珠溶液经心脏灌流，随后分离肾小球，提取RNA，并通过Affymetrix外显子芯片进行差异表达分析。