The Transcriptomic Footprint of Mytella strigata: A De Novo Transcriptome Assembly of a Major Invasive Species

Mytella strigata, a potentially invasive species native to South America, is rapidly spreading across various aquatic ecosystems around the globe, posing a threat to native mussels. This study presents the first comprehensive de novo transcriptome assembly of M. strigata. We generated 254 million reads which were processed and assembled using the Trinity assembler, resulting in an N50 of 1,578 bp and over 93-98% completeness, as confirmed by BUSCO analysis with multiple orthodatasets. A number of databases were used for functional annotation, including UniProt, KEGG, Reactome, InterPro, and eggNOG. Gene Ontology and pathway analyses identified transcripts associated with key biological processes, including those associated with cell signalling, metabolism, stress responses, cancer pathways, and immune regulation. This dataset enriches the bivalve database by advancing the understanding of the adaptive success and evolutionary resilience of this invasive species. The present study provides a fundamental framework for future research on the ecological and evolutionary impacts of this invasive species.

条纹贻贝（Mytella strigata）是一种原产于南美洲的潜在入侵物种，目前正快速在全球各类水生生态系统中扩散，对本土贻贝种群构成威胁。本研究首次完成了该物种的全流程从头转录组组装。本研究共生成2.54亿条测序读段，经质控处理后使用Trinity组装软件（Trinity assembler）完成组装，最终得到N50值为1578 bp的转录组，经基于多套直系同源数据集的BUSCO评估（Benchmarking Universal Single-Copy Orthologs）确认，其组装完整度达93%~98%。本研究采用多个数据库开展功能注释，包括UniProt、KEGG、Reactome、InterPro及eggNOG。基因本体（Gene Ontology）与通路富集分析鉴定出与多个关键生物学过程相关的转录本，涵盖细胞信号传导、新陈代谢、应激反应、癌症通路及免疫调控等过程。本数据集通过加深对该入侵物种适应性成功与进化韧性的理解，丰富了双壳类数据库资源。本研究为后续探究该入侵物种的生态与进化影响提供了基础研究框架。